The Bluebirds have been drawn to host Ammanford in Round One of the Nathaniel MG Cup.
The fixture against the Cymru South side will take place between the 18th and 20th of July.
City reached the Semi-Finals of the competition last year and gained invaluable experience of facing men's sides.
After entering the competition as a wildcard again for the 2025/26 campaign, Academy Manager Gavin Chesterfield said: "It's extremely valuable. What we're aiming to do is to prepare players for a senior environment. When the time is right, that may include a loan or experience with the First Team. The Nathaniel MG Cup has provided us with exposure to real-life scenarios with jeopardy on the result as a knockout game.
"We were grateful for the experiences that the boys went through last year and to lose in the Semi-Finals on penalties, it was a part of their learning curves in their journeys.
"All the clubs in the competition are part of the Welsh football ecosystem. For us as Cardiff City Football Club, I think it's a really important part of our responsibility to connect with those local clubs."